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KMID : 1025520080500040509
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Journal of Animal Science and Technology
2008 Volume.50 No. 4 p.509 ~ p.518
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Characterization of Lactobacilli Lsolated from Chicken Ceca as Probiotics
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Kim Sang-Ho
Kim Dong-Wook
Park Su-Young
Kim Ji-Hyuk
Kang Geun-Ho
Kang Hwan-Ku
Yu Dong-Jo
Na Jae-Cheon
Lee Sang-Jin
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Abstract
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This experiment was conducted to investigate enzyme activity, antimicrobial activity, and antibiotics susceptibility of Lactobacilli strain(Lactobacillus reuteri BLA5, Lactobacillus crispatus BLA7, Lactobacillus reuteri BLA9, Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9, Lactobacillus vaginalis LLA11) isolated from chicken ceca and were selected by organic acid synthesis, acid tolerance, bile salt tolerance. The enzymes activities were different among strains of Lactobacilli. The amylase activity and lipase activity of Lactobacillus were high but cellulase activity and protease activity of that were low. Lactobacillus culture showed high antimicrobial activity against E. coli but low antimicrobial activity against Salmonella. The inhibitory factor of Lactobacilli isolated from chickens’ cecum on E. coli was low pH by organic acid. All of Lactobacillus isolated from chicken’s cecum were susceptible to ampicillin and amoxicillin but weren’t susceptible at the optimum level of feed additive antibiotics(virginiamycin and salinomycin).
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KEYWORD
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Lactobacillus, Enzyme activity, Antimicrobial activity, Antibiotics susceptibility
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